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US 2006/0134695 A1Published application
Biosensor surface structures and methods
AssigneeSensata Technologies, Inc.
InventorJohn Quinn
Life of this patent
Estimating the term…
Abstract
A biosensor surface with a low density of ligand-carrying tether molecules on a base layer. Also, surface plasmon resonance (SPR) devices have the biosensor surface attached to a thin gold layer with backside angle-spread incident radiation for resonance excitation and reflective detection.
Claims12
A sensing surface comprising:
- (a) a planar base layer on a;
- and
- (b) ligands coupled to said base layer;
- (c) wherein the surface of said base layer contains charged groups for bringing about a concentration of oppositely charged ligand molecules at said surface and reactive groups for covalent coupling to ligands and wherein said ligands optionally possess one or more tether molecules of a length exceeding 1 nm wherein said tether molecules contain at least one reactive group that forms an irreversible covalent bond with said base layer reactive groups.
1 more element
A process of fabricating a sensing surface, comprising the steps of:
- (a) forming a base layer, said base layer is composed of a monolayer of colloidal particles chosen from a group consisting of proteins, peptides, colloidal PTFE particles, colloidal polyethylene particles, colloidal polypropylene particles wherein said particles are of a diameter in the range of 0.5 nm to 100 nm;
- (b) epoxy-group activating said monolayer by covalent coupling of a monolayer of a bisoxirane, said epoxy-group activated monolayer is of a thickness not to exceed 1 nm;
- (c) reacting a fraction of said epoxy groups with thioglycolate to incorporate carboxyl groups and the remaining fraction are hydrolyzed to yield hydroxyl groups;
- and
- (d) binding ligands to said monolayer.
2 more elements
An SPR biosensor, comprising:
- (a) a base layer bound to a plasmon resonance capable metal film, said base layer composed of a monolayer of colloidal particles chosen from a group consisting of proteins, peptides, colloidal PTFE particles, colloidal polyethylene particles, and colloidal polypropylene particles, said particles are of a diameter in the range of 0.5 nm to 100 nm;
- (b) ligands bound to said base layer;
- and
- (c) an optical source and reflection detector optically coupled to said metal film;
- (d) wherein said monolayer of particles is epoxy group activated by covalent coupling of a monolayer of a bisoxirane, said epoxy-activated monolayer is of a thickness not to exceed 1 nm.
2 more elements
Dependencies are inferred from the claim text.