Methods of protein profiling by thiolation
AssigneeIntel Corporation
InventorMineo Yamakawa
Life of this patent
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Abstract
Methods for analyzing a protein are provided, including isolating the protein having original reactive functional groups from a biological sample, and converting substantially all original reactive functional groups into thiol groups, followed by attaching tags to the protein via the thiol groups.
Claims63
A method for modifying a protein, comprising:
- (a) isolating the protein having original reactive non-thio functional groups from a biological sample;
- and
- (b) converting the original reactive non-thio functional groups into thiol groups to obtain a thiolized protein thereby, wherein the thiolized protein contains less than about 2% (mol) of the original reactive non-thio functional groups.
A composition, comprising an identifying moiety bonded to a thiolized protein, wherein the thiolized protein contains less than about 2% of reactive non-thio functional groups.
A composition, comprising a thiolized protein, wherein the thiolized protein contains less than about 2% of non-thio reactive functional groups.
Dependencies are inferred from the claim text.