Process for the purification of interleukin-4 and its muteins
OwnerJorg Peters, Torsten Minuth +1inventors
InventorsJorg Peters, Torsten Minuth, Hans-Georg Dellweg
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Abstract
This invention relates generally to a method for purifying Interleukin-4 or related variants from an Escherichia coli culture medium, where Interleukin-4 and its derivatives are expressed as insoluble protein aggregates, so-called inclusion bodies.
Claims10
A method for preparation of Interleukin-4 or muteins of Interleukin-4 by recombinant expression comprising (a) expression in inclusion bodies, (b) disrupting the cells and separating the inclusion bodies, (c) washing inclusion bodies so obtained, (d) solubilizing the inclusion bodies by denaturation (e) renaturating the expression product and (f) purifying the expression product characterized in that the inclusion bodies are washed (c) with a buffer containing a detergent which effectively solubilizes lipids bound to the surface of the inclusion body or lipids contained in cell wall fragments.
Dependencies are inferred from the claim text.