Real-time polymerase chain reaction-based genotyping assay for single nucleotide polymorphism
AssigneeUniversity of Tennessee Research Foundation
InventorsCharles Yates, Duane Miller, Dick Gourley, Pengfei Song
Life of this patent
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Abstract
The present invention provides fluorescence-based real-time PCR assays for the rapid detection of single nucleotide polymorphisms (SNPs). The genotyping assay can be used to detect SNPs of a number of genes of interest that include, but are not limited to, the human multidrug resistance gene (MDR1) single nucleotide polymorphisms C3435T and G2677T, and cytochrome P-450 3A5 single nucleotide polymorphisms CYP3A5*3 (A22893G) and CYP3A5*6 (G30597A).
Claims15
An isolated DNA molecule useful as a primer for genotyping human multidrug resistance gene (MDR1) single nucleotide polymorphism G2677T, said DNA is selected from the group consisting of SEQ ID NOs:
- 4-6.
An isolated DNA molecule useful as a primer for genotyping human cytochrome P-450 3A5 single nucleotide polymorphism CYP3A5*3, said DNA is selected from the group consisting of SEQ ID NOs:
- 11-13.
An isolated DNA molecule useful as a primer for genotyping human cytochrome P-450 3A5 single nucleotide polymorphism CYP3A5*6, said DNA is selected from the group consisting of SEQ ID NOs:
- 14-16.
Dependencies are inferred from the claim text.