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US 2006/0134686 A1Published application
Unary avian myeloblastosis virus reverse transcriptase and its use
AssigneeTosoh Corporation
InventorsKiyoshi Yasukawa, Takahiko Ishiguro
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Abstract
An avian myeloblastosis virus reverse transcriptase composition which comprises a heterodimer consisting of an α subunit having a molecular weight of 68000 and a β subunit having a molecular weight of 92000 in an amount of at least 80%.
Claims2
1-5. (canceled)
A method of amplifying a target RNA molecule comprising:
- (a) hybridizing a first DNA primer to a target RNA molecule,
- (b) elongating the first DNA primer to form a DNA-RNA heteroduplex molecule,
- (c) degrading the target RNA molecule present in the DNA-RNA heteroduplex molecule of (b) to form a DNA molecule,
- (d) hybridizing a second DNA primer to the DNA molecule of (c),
- (e) elongating the second DNA primer to form a double-stranded DNA molecule,
- (f) transcribing the double-stranded DNA molecule of (e) to form an RNA molecule, wherein either the first or second DNA primer has a promoter region, and wherein said elongation of (b), said degradation of (c), and said elongation of (e) is performed by a polypeptide heterodimer consisting of an avian myeloblastosis virus (AMV) reverse transcriptase α subunit and an AMV reverse transcriptase β subunit.
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Dependencies are inferred from the claim text.