Homogeneous populations of nucleic acids, methods of synthesyzing same and uses thereof

A homogeneous population of fully double stranded nucleic acid molecules having blunt ends, each of said molecules in said population being at least 1000 base pairs long, each of said molecules in said population having the same repetitive core sequence of an A mononucleotide.

A method of synthesizing a homogeneous population of fully double stranded nucleic acid molecules having blunt ends, the method comprising reacting a fully double stranded initiator nucleic acid molecule having blunt ends and further having a repetitive core sequence selected from the group consisting of mono-, di-, or tri-nucleotide with nucleotide tri phosphates in the presence of a nucleic acid polymerase, thereby de novo enzymatically synthesizing the homogeneous population of fully double stranded nucleic acid molecules having blunt ends.

A kit for de novo synthesizing a homogeneous population of nucleic acid molecules, the kit comprising, in a single container nucleotides and fully double stranded initiator nucleic acid molecules, said fully double stranded initiator nucleic acid molecules having blunt ends and further having a repetitive core sequence selected from the group consisting of mono-, di-, or tri-nucleotide.