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US 2006/0134679 A1Published application
Methods and compositions for acquiring information from unstretched polymer conformations
AssigneeU.S. Genomics, Inc.
InventorJonathan Larson
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Abstract
The invention relates to the methods for analyzing polymers that are in unstretched conformations. In particular, the polymers such as nucleic acids may be present in hairpin conformations.
Claims21
A method for analyzing a polymer in an unstretched conformation comprising
- identifying a single hairpin polymer in a polymer population based on a measured length that is shorter than a contour length, and
- comparing a probe binding profile of the single hairpin polymer to a hairpin dataset,
- wherein the polymer is labeled with a backbone label.
A method for analyzing a polymer in an unstretched conformation comprising
- determining total integrated backbone label signal intensity of a polymer as an indicator of contour length wherein the polymer is labeled with a backbone label,
- determining measured length based on transit time of the polymer between two positions wherein the polymer is traveling at a known velocity,
- comparing contour length and measured length wherein a measured length that is shorter than a contour length is indicative of an unstretched polymer,
- determining a probe binding profile of the unstretched polymer, and
- processing the probe binding profile.
2 more elements
Dependencies are inferred from the claim text.