Plasmon resonance biosensor and method
AssigneePlexera LLC
InventorVincenzo Casasanta
Life of this patent
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Abstract
A method of analyzing a sample comprising directing a beam of light to a spot on the surface of a microarray treated with the sample using a digital micromirror device, and observing the SPR spectral shift due to a chemical binding event. The digital micromirror device can be, for example, that used in Digital Light Processing (DLP). Such a device can selectively place a pixel of light onto a microarray such that each spot can be observed at millisecond intervals and the whole microarray can be sequentially scanned over a relatively short period. The SPR spectral shift for each spot can be measured as a function of time, thus producing SPR detection of molecular binding in an array format.
Claims25
A method for analyzing a sample comprising:
- (a) providing a microarray comprising a surface having a plurality of discrete locations, each of which includes a first member of a chemical binding pair and is associated with a surface plasmon resonance;
- (b) irradiating the discrete locations on the surface of the microarray with a light beam using an apparatus comprising a light source, a digital micromirror device, and a prism or lens located between the digital micromirror device and the surface of the microarray;
- (c) contacting the surface of the microarray with a sample comprising a second member of the chemical binding pair, whereupon, in at least one of the discrete locations, the second member of the chemical binding pair binds to the first member of the chemical binding pair on the surface of the microarray and causes a spectral shift in the surface plasmon resonance associated with the discrete location where the chemical binding occurs;
- and
- (d) detecting the surface plasmon resonance spectral shift associated with each location as a function of time.
A method, comprising
- a) providing a substrate having a first surface and a second surface, the first surface having a thin metal film thereupon, the thin metal film having a surface opposed to the interface of the thin metal film and the first surface;
- b) generating surface plasmon resonance in predetermined areas on the surface of the thin metal film with light that is directed at the interface by a digital micromirror device;
- c) generating a chemical binding event near the surface of the thin metal film;
- and
- d) detecting a spectral shift of the surface plasmon resonance due to the chemical binding event near the surface of the thin metal film.
Dependencies are inferred from the claim text.