Method for detecting fusion gene
OwnerMasatoshi Narahara, Toshiro Saito +2inventors
InventorsMasatoshi Narahara, Toshiro Saito, Takayuki Obara, Kenji Yasuda
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for detecting fusion gene transcripts resulting from chromosomal translocation. Specifically, the method of the present invention comprises allowing at least two or more probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or complementary base sequence thereof, and each of which immobilized on a support, to hybridize with a sample containing a nucleic acid derived from a fusion gene, thereby allowing the detection of two or more fusion genes at a time.
Claims11
A method for detecting fusion gene transcripts wherein the method comprises allowing at least two or more of probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or complementary base sequence thereof, and each of which is immobilized on a support, to hybridize with a sample containing a nucleic acid derived from a fusion gene, thereby allowing the detection of two or more fusion genes at a time.
A kit for detecting fusion gene transcripts comprising the following (a) to (c):
- (a) a support on which two or more probes, each of which contains a partial base sequence of the exons which sandwich the breakpoint of a fusion gene or complementary base sequences thereof are immobilized;
- (b) a first primer containing a base sequence complementary to a base sequence of at least ten or more contiguous bases which exist on the 3′ side from the breakpoint of the fusion gene;
- and
- (c) a second primer containing a base sequence of at least ten or more contiguous bases which exist on the 5′ side from the breakpoint of the fusion gene.
Dependencies are inferred from the claim text.