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US 2006/0134660 A1Published application
Alien sequences
AssigneesModular Genetics, Inc., Trustees of Boston University
InventorsSean Quinlan, Temple Smith, Prashanth Vishwanath
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Abstract
The present invention provides sequences and reagents for preparing microarrays with internal controls. Specifically, the present invention defines and provides sequences that are not present in the hybridizing mRNA or cDNA, and therefore can be used both as hybridization controls and for inter-spot normalization.
Claims13
A nucleic acid array comprising:
- a solid support
- a plurality of nucleic acid probes attached to the solid support at discrete locations, wherein at least one of the probes is an alien probe in that it has a sequence that is alien to a hybridizing mixture to be hybridized to the array.
A method comprising steps of:
- providing a hybridizing mixture comprising a plurality of nucleic acids;
- and
- hybridizing the hybridizing mixture to a nucleic acid array comprising:
- a solid support;
- and
- a plurality of nucleic acid probes attached to the solid support at discrete locations, wherein at least one of the probes is an alien probe in that it has a sequence that is alien to a hybridizing mixture to be hybridized to the array.
3 more elements
Dependencies are inferred from the claim text.