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US 2006/0134655 A1Published application
Method of detection and quantification of cGMP by using a cGMP-visualizing probe
AssigneeJapan Science and Technology Corporation
InventorsYoshio Umezawa, Moritoshi Sato, Takeaki Ozawa
Life of this patent
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Abstract
As a cGMP-visualizing probe capable of detecting and quantifying cGMP easily and accurately even in vivo and for a method of detecting and quantifying cGMP by using the same, a cGMP-visualizing probe comprising a polypeptide, which binds specifically to cGMP, and two chromophores having different fluorescence wavelengths each linked respectively to the two terminals of said polypeptide is provided.
Claims6
1-5. (canceled)
A method for detecting and quantifying cGMP, which comprises:
- introducing a polynucleotide expressing a cGMP-visualizing probe into cells, whereby the cGMP-visualizing probe is made to coexist with cGMP, wherein the cGMP-visualizing probe comprises a polypeptide that binds specifically to cGMP and two chromophores with different fluorescence wavelengths, which are linked separately to the two terminals of the polypeptide, wherein the chromophores are capable of exhibiting fluorescence resonance energy transfer (FRET);
- and
- measuring the change in fluorescence wavelength.
7-9. (canceled)
Dependencies are inferred from the claim text.