Inhibiting the growth of bacterial biofilms
AssigneeInstitut Pasteur
InventorsJean-Marc Ghigo, Christophe Beloin, Patricia Latour-Lambert
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Abstract
The present invention provides targets and methods for inhibiting the development, formation and/or maturation of bacterial biofilms, and for detecting bacterial biofilms.
Claims58
A method for identifying a substance which inhibits the formation of a bacterial biofilm, comprising
- providing a host cell expressing at least one protein encoded by a gene selected from the group consisting of lctR, recA, mdh, rbsB, msrA, finA, tatE, pspF, cpxP, spy, ycfJ, ycfR, yoaB, yqcc, yggN, ymcA, yccA, yfcx, yghO, ycP, and ycuB;
- contacting the host cell with the substance;
- measuring the level of at least one protein or at least one RNA transcript of the at least one gene after said contacting;
- and
- comparing the level of the at least one of the protein or RNA transcript of the at least one gene after said contacting with a host cell not contacted with the substance;
- wherein a reduced level of the at least one protein or the at least one RNA transcript relative to the cell not contacted with the substance indicates that the substance inhibits the formation of a bacterial biofilm.
A method for detecting differentially expressed polynucleotide sequences which are specifically correlated with a mature bacterial biofilm, said method comprising:
- obtaining a polynucleotide sample;
- labeling said polynucleotide sample by reacting said polynucleotide sample with a labeled probe immobilized on a solid support wherein said probe comprises at least one polynucleotide sequence selected from the group consisting of lctR, recA, mdh, rbsB, msrA, finA, tatE, pspF, cpxP, spy, ycfJ, ycfR, yoaB, yqcC, yggN, ymcA, yccA, yfcx, yghO, ycP, and ycuB or an expression product encoded by any of the polynucleotide sequences;
- and detecting a polynucleotide sample reaction product.
A method of detecting significantly overexpressed genes correlated with a mature bacterial biofilm comprising detecting at least one polynucleotide sequence or subsequence of a polynucleotide selected from the group consisting of lctR, recA, mdh, rbsB, msrA, finA, tatE, pspF, cpxP, spy, ycfJ, ycfR, yoaB, yqcC, yggN, ymcA, yccA, yfcx, yghO, ycP, and ycuB or detecting at least one product encoded by said polynucleotide library in a sample obtained from a patient.
A polynucleotide library useful in the molecular characterization of a mature bacterial biofilm, said library comprising a pool of polynucleotide sequences or subsequences thereof wherein said sequences or subsequences are overexpressed in mature bacterial biofilms, further wherein said sequences or subsequences correspond substantially to one or more polynucleotide sequences selected from the group consisting of rne, lctR, dinI, glpQ, mdh, sixA, lamB, rbsB, gadA, pspA, pspB, pspC, pspD, tatE, cpxP, rseA, rpoE, spy, yebE, yqcC, yfcX, yjbO, yceP, and ygiB.
The polynucleotide library of 51, wherein said one or more polynucleotide sequences or subsequences of said pool are immobilized on a solid support to form a polynucleotide array.
Dependencies are inferred from the claim text.