Method for the detection of cancer related gene transcripts in blood
AssigneeChondroGene Limited
InventorChoong-Chin Liew
Life of this patent
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Abstract
The present invention is directed to detection and measurement of gene transcripts and their equivalent nucleic acid products in blood. Specifically provided is analysis performed on a drop of blood for detecting, diagnosing and monitoring diseases using gene-specific and/or tissue-specific primers. The present invention also describes methods by which delineation of the sequence and/or quantitation of the expression levels of disease-specific genes allows for an immediate and accurate diagnostic/prognostic test for disease or to assess the effect of a particular treatment regimen.
Claims52
A method of identifying one or more markers for cancer, wherein each of said one or more markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having cancer, wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for cancer;
- and
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals not having cancer,
- wherein those compared transcripts which display differing levels in the comparison of step b) are identified as being markers for cancer.
A method of identifying one or more markers for cancer, wherein each of said one or more markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having cancer, wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for cancer;
- and
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals having cancer,
- wherein those compared transcripts which display the same levels in the comparison of step b) are identified as being markers for cancer.
A method of identifying one or more markers of a stage of cancer progression or regression, wherein each of said one or more markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having a stage of cancer, wherein said one or more individuals are at the same progressive or regressive stage of cancer, and wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for determining the stage of progression or regression of cancer, and;
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals who are at a progressive or regressive stage of cancer distinct from that of said one or more individuals of step a),
- wherein those compared transcripts which display differing levels in the comparison of step b) are identified as being markers for the stage of progression or regression of cancer.
A method of identifying one or more markers of a stage of cancer progression or regression, wherein each of said one or more markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having a stage of cancer, wherein said one or more individuals are at the same progressive or regressive stage of cancer, and wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for determining the stage of progression or regression of cancer, and;
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals who are at a progressive or regressive stage of cancer identical to that of said one or more individuals of step a),
- wherein those compared transcripts which display the same levels in the comparison of step b) are identified as being markers for the stage of progression or regression of cancer.
A plurality of nucleic acid molecules that identify or correspond to two or more genes selected from the group consisting of the genes listed in Table 3J, Table 3K and Table 3×.
Dependencies are inferred from the claim text.