Method for the detection of coronary artery disease related gene transcripts in blood
AssigneeChondroGene Limited
InventorChoong-Chin Liew
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Abstract
The present invention is directed to detection and measurement of gene transcripts and their equivalent nucleic acid products in blood. Specifically provided is analysis performed on a drop of blood for detecting, diagnosing and monitoring diseases using gene-specific and/or tissue-specific primers. The present invention also describes methods by which delineation of the sequence and/or quantitation of the expression levels of disease-specific genes allows for an immediate and accurate diagnostic/prognostic test for disease or to assess the effect of a particular treatment regimen.
Claims44
A method of identifying one or more genetic markers for coronary artery disease, wherein each of said one or more genetic markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having coronary artery disease, wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for coronary artery disease;
- and
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals not having coronary artery disease,
- wherein those compared transcripts which display differing levels in the comparison of step b) are identified as being genetic markers for coronary artery disease.
A method of identifying one or more genetic markers for coronary artery disease, wherein each of said one or more genetic markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having coronary artery disease, wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for coronary artery disease;
- and
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals having coronary artery disease,
- wherein those compared transcripts which display the same levels in the comparison of step b) are identified as being genetic markers for coronary artery disease.
A method of identifying one or more genetic markers of a stage of coronary artery disease progression or regression, wherein each of said one or more genetic markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having a stage of coronary artery disease, wherein said one or more individuals are at the same progressive or regressive stage of coronary artery disease, and wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for determining the stage of progression or regression of coronary artery disease, and;
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals who are at a progressive or regressive stage of coronary artery disease distinct from that of said one or more individuals of step a),
- wherein those compared transcripts which display differing levels in the comparison of step b) are identified as being genetic markers for the stage of progression or regression of coronary artery disease.
A method of identifying one or more genetic markers of a stage of coronary artery disease progression or regression, wherein each of said one or more genetic markers corresponds to a gene transcript, comprising the steps of:
- a) determining the level of one or more gene transcripts expressed in blood obtained from one or more individuals having a stage of coronary artery disease, wherein said one or more individuals are at the same progressive or regressive stage of coronary artery disease, and wherein each of said one or more transcripts is expressed by a gene that is a candidate marker for determining the stage of progression or regression of coronary artery disease, and;
- b) comparing the level of each of said one or more gene transcripts from said step a) with the level of each of said one or more genes transcripts in blood obtained from one or more individuals who are at a progressive or regressive stage of coronary artery disease identical to that of said one or more individuals of step a),
- wherein those compared transcripts which display the same levels in the comparison of step b) are identified as being genetic markers for the stage of progression or regression of coronary artery disease.
A method of diagnosing or prognosing coronary artery disease in an individual, comprising the steps of:
- a) determining the level of one or more gene transcripts in blood obtained from said individual suspected of having coronary artery disease, and
- b) comparing the level of each of said one or more gene transcripts in said blood according to step a) with the level of each of said one or more gene transcripts in blood from one or more individuals not having coronary artery disease,
- wherein detecting a difference in the levels of each of said one or more gene transcripts in the comparison of step b) is indicative of coronary artery disease in the individual of step a).
A method of diagnosing or prognosing coronary artery disease in an individual, comprising the steps of:
- a) determining the level of one or more gene transcripts in blood obtained from said individual suspected of having coronary artery disease, and
- b) comparing the level of each of said one or more gene transcripts in said blood according to step a) with the level of each of said one or more gene transcripts in blood from one or more individuals having coronary artery disease,
- wherein detecting the same levels of each of said one or more gene transcripts in the comparison of step b) is indicative of coronary artery disease in the individual of step a).
A method of determining a stage of disease progression or regression in an individual having coronary artery disease, comprising the steps of:
- a) determining the level of one or more gene transcripts in blood obtained from said individual having coronary artery disease, and
- b) comparing the level of each if said one or more gene transcripts in said blood according to step a) with the level of each of said one or more gene transcripts in blood obtained from one or more individuals who each have been diagnosed as being at the same progressive or regressive stage of coronary artery disease,
- wherein the comparison from step b) allows the determination of the stage of coronary artery disease progression or regression in an individual.
A kit for diagnosing or prognosing coronary artery disease comprising:
- a) two gene-specific priming means designed to produce double stranded DNA complementary to a gene selected from the group consisting of Table 3L;
- wherein said first priming means contains a sequence which can hybridize to RNA, cDNA or an EST complementary to said gene to create an extension product and said second priming means capable of hybridizing to said extension product;
- b) an enzyme with reverse transcriptase activity c) an enzyme with thermostable DNA polymerase activity and d) a labeling means;
- wherein said primers are used to detect the quantitative expression levels of said gene in a test subject.
A kit for monitoring a course of therapeutic treatment of coronary artery disease, comprising a) two gene-specific priming means designed to produce double stranded DNA complementary to a gene selected group consisting of Table 3L;
- wherein said first priming means contains a sequence which can hybridize to RNA, cDNA or an EST complementary to said gene to create an extension product and said second priming means capable of hybridizing to said extension product;
- b) an enzyme with reverse transcriptase activity c) an enzyme with thermostable DNA polymerase activity and d) a labeling means;
- wherein said primers are used to detect the quantitative expression levels of said gene in a test subject.
A kit for monitoring progression or regression of coronary artery disease, comprising:
- a) two gene-specific priming means designed to produce double stranded DNA complementary to a gene selected group consisting of Table 3L;
- wherein said first priming means contains a sequence which can hybridize to RNA, cDNA or an EST complementary to said gene to create an extension product and said second priming means capable of hybridizing to said extension product;
- b) an enzyme with reverse transcriptase activity c) an enzyme with thermostable DNA polymerase activity and d) a labeling means;
- wherein said primers are used to detect the quantitative expression levels of said gene in a test subject.
A plurality of nucleic acid molecules that identify or correspond to two or more sequences from Table 3L.
Dependencies are inferred from the claim text.