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US 2006/0134631 A1Published application
Novel DNA glycosylases and their use
OwnerHans Krokan, Geir Slupphaug +3inventors
InventorsHans Krokan, Geir Slupphaug, Bodil Kavli, John Tainer, Clifford Mol
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Abstract
Novel cytosine-, thymine- and uracil-DNA glycosylases, subcellular localization peptides, nucleic acid molecules containing the same, methods of identifying such enzymes and their use in various methods including mutagenesis, cell killing and DNA sequencing and modification, are desired.
Claims18
An isolated thymine-DNA glycosylase (TDG) capable of releasing thymine bases from single stranded (ss) DNA and double stranded (ds) DNA,
- said TDG obtainable by modification of a uracil-DNA glycosylase (UDG), wherein tyrosine (Tyr) at amino acid position 147 is substituted or modified in the human UDG protein encoded
- a) by the human uracil nucleic acid glycosylase gene (UNG1) (SEQ ID NO:
- 8), or
- b) by the alternatively spliced human uracil nucleic acid glycosylase gene (UNG2) (SEQ ID NO:
- 2), or
- wherein an equivalent residue in a homologous UDG of another species is substituted or modified.
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