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OwnerHilde De Henau, Joachim Van Crombruggen +5inventors
InventorsHilde De Henau, Joachim Van Crombruggen, Geert Jannes, Thomas Emrich, Geert Jannes, Gerd Haberhausen, Thomas Emrich
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The present invention relates to a method for the specific detection and/or identification of Enterococcus species, in particular Enterococcus faecalis and/or Enterococcus faecium, using new nucleic acid sequences derived from the ITS (internal Transcribed Spacer) region. The present invention relates also to said new nucleic acid sequences derived from the ITS region, between the 16S and 23S ribosomal ribonucleic acid (rRNA) or rRNA genes, to be used for the specific detection and/or identification of Enterococcus species, in particular of Enterococcus faecalis and/or Enterococcus faecium, in a biological sample. It relates also to nucleic acid primers to be used for the amplification of said spacer region of Enterococcus species in a sample.
An isolated nucleic acid molecule consisting of SEQ ID NO 1, its complementary form and the RNA form thereof.
An isolated nucleic acid molecule consisting of SEQ ID NO 2, its complementary form and the RNA form thereof.
An isolated nucleic acid molecule that specifically hybridizes to SEQ ID NO 1 or 2, or to the RNA form of said SEQ ID NO 1 or 2 wherein T is replaced by U, or to the complementary form of said SEQ ID NO 1 or 2, or to a fragment of at least 20 contiguous nucleotides thereof, or to any of their homologues, for the detection and/or identification of Enterococcus species, in particular of E. faecalis and/or E. faecium.
A set of two or three polynucleotide probes, said probes hybridizing specifically to SEQ ID NO 1 or SEQ ID NO 2 or homologues, or to their RNA form wherein T is replaced by U, or to their complementary form, wherein there are no more than 25 nucleotides between said probes.
Use of a nucleic acid molecule consisting of SEQ ID NO 1 or 2, or of the RNA form of said SEQ ID NO 1 or 2 wherein T is replaced by U, or of the complementary form of said SEQ ID NO 1 or 2, or of a fragment of at least 20 contiguous nucleotides thereof, or of any of their homologues, for the detection and/or identification of Enterococcus species, in particular of E. faecalis and/or E. faecium.
Dependencies are inferred from the claim text.