Screens for altered immune response capability
AssigneeThe Edward Jenner Institute for Vaccine Research
InventorsPeter Beverly, Elma Tchilian, Tara Stanton, Walter Bodmer
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Abstract
The invention relates to associations between genetic variation in the gene encoding CD45 and human disease and human immune responses. In particular the invention provides methods of screening human subjects for the presence of an “altered immune response capability”, which may in turn affect susceptibility to viral disease and/or autoimmune disease.
Claims32
A method of screening a human subject for susceptibility to viral infection and/or pre-disposition to developing severe disease following viral infection, which method comprises screening for the presence or absence in the genome of the subject of one or more polymorphic variants or mutations in the gene encoding CD45 or of one or more polymorphic variants in linkage disequilibrium with or in close physical proximity to a polymorphic locus in the gene encoding CD45.
4-5. (canceled)
A method of screening a human subject for an altered immune response capability, which method comprises screening for the presence or absence in said subject of a mutation in the gene encoding CD45, which mutation is characterised in that subjects carrying at least one mutant allele exhibit altered CD45 splicing resulting in an increase in the proportion of the T cell population carrying the CD45R0 splice variant but lacking CD45RA expression as compared to subjects not carrying a mutant allele, wherein subjects having at least one mutant allele are scored as having altered immune response capability.
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20-21. (canceled)
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25-26. (canceled)
A method of screening a human subject for susceptibility to viral infection and/or pre-disposition to developing severe disease following viral infection which comprises evaluating the pattern of CD45 mRNA expression in the subject or evaluating the pattern of CD45 protein expression in the subject, wherein the presence of an abnormal pattern of CD45 mRNA expression characterised by reduced splicing out of exon 4 of the CD45 mRNA and a quantitative decrease in amount of CD45RO transcript, or the presence of an abnormal pattern of CD45 protein expression characterised as a reduction in the proportion of T lymphocytes expressing the CD45R0 isoform but lacking CD45RA expression is taken as an indication that the subject is susceptible to viral infection and/or pre-disposed to developing severe disease following viral infection.
(canceled)
A method of screening a human subject for susceptibility to viral infection and/or pre-disposition to developing severe disease following viral infection which comprises evaluating the pattern of CD45 mRNA expression or the pattern of CD45 protein expression in the subject, wherein the presence of an abnormal pattern of CD45 mRNA expression characterised by a quantitative increase in the level of expression of the CD45R0 transcript, or the presence of abnormal pattern of CD45 protein expression characterised by an increase in the proportion of T lymphocytes expressing the CD45R0 isoform but lacking CD45RA expression is taken as an indication that the subject is not susceptible to viral infection and/or is pre-disposed to developing less severe disease following viral infection.
A method of screening a human subject for an altered immune response capability, which method comprises evaluating the pattern of CD45 mRNA expression in said individual, wherein the presence of an abnormal pattern of CD45 mRNA expression characterised by a quantitative increase in the level of expression of the CD45R0 transcript is taken as an indication that the subject has an altered immune response capability. and/or pre-disposed to developing less severe disease following viral infection, as compared to subjects who do not carry a 138G mutant allele.
A method of screening a human subject for an altered immune response capability, which method comprises evaluating the pattern of CD45 mRNA expression in said individual, wherein the presence of an abnormal pattern of CD45 mRNA expression characterised by a quantitative increase in the level of expression of the CD45R0 transcript is taken as an indication that the subject has an altered immune response capability.
35-52. (canceled)
Dependencies are inferred from the claim text.