Chemically amplified electrochemical detection of affinity reaction
OwnerLianghong Guo, Yuanguang Li +4inventors
InventorsLianghong Guo, Yuanguang Li, Na Wang, Fuquan Wang, Xiqiang Yang, Jing Cheng
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Abstract
This invention relates generally to the field of electrochemistry. In particular, the invention provides a method for assaying an analyte, using, inter alia, a reactant capable of binding and/or reacting with an analyte to be analyzed on an oxide electrode and a reducing agent not capable of being oxidized directly by said electrode to generate reduced electrochemically active molecule that participates in oxidation-reduction reactions repeatedly to generate an amplified electrochemical signal to determine presence and/or amount of said analyte in said sample.
Claims55
A method for assaying an analyte, which method comprises:
- a) providing a reactant capable of binding and/or reacting with an analyte to be analyzed on an oxide electrode;
- b) contacting a sample suspected of containing said analyte with said reactant provided in step a) under suitable conditions to allow binding of said analyte, if present in said sample, to said reactant, wherein said reactant, said analyte, or additional reactant or additional analyte or analyte analog is covalently linked to an electrochemically active molecule in a reduced form, and said contacting brings said electrochemically active molecule into close proximity to said electrode to allow oxidation of said electrochemically active molecule by said electrode;
- c) allowing reduction of said oxidized electrochemically active molecule back to said reduced form by a reducing agent, wherein said reducing agent is not capable of being oxidized directly by said electrode, and said reduced electrochemically active molecule participates in said oxidation-reduction reactions of steps b) and c) repeatedly to generate an amplified electrochemical signal;
- and
- d) assessing said amplified electrochemical signal to determine presence and/or amount of said analyte in said sample.
A kit for assaying an analyte, which kit comprises:
- a) a reactant capable of binding and/or reacting with an analyte to be analyzed on an oxide electrode;
- b) an additional reactant, analyte, or analyte analog that is covalently linked to an electrochemically active molecule in a reduced form, wherein contacting of said analyte with said reactant on said electrode in the presence of said additional reactant, analyte, or analyte analog that is covalently linked to said electrochemically active molecule brings said electrochemically active molecule into close proximity to said electrode to allow oxidation of said electrochemically active molecule by said electrode;
- c) a reducing agent, wherein said reducing agent is not capable of being oxidized directly by said electrode, and said reducing agent reduces said oxidized electrochemically active molecule back to said reduced form to participate in repeated oxidation-reduction reactions to generate an amplified electrochemical signal;
- and
- d) means for assessing said amplified electrochemical signal to determine presence and/or amount of said analyte in said sample.
Dependencies are inferred from the claim text.