Flexible method and apparatus for high throughput production and purification of multiple proteins

An apparatus for purification of a plurality of biological substances, comprising:

• a first reservoir having a first solution including a biological substance disposed therein;
• a second reservoir having a second solution including a biological substance disposed therein;
• a first valve, said first reservoir being connected to said first valve;
• a second valve, said second reservoir being connected to said second valve;
• a first column connected for fluid communication to said first valve directing the first solution through said first column, said first column having a biological substance retaining material disposed therein;
• a second column connected for fluid communication to said second valve directing the second solution through said second column, said second column having a biological substance retaining material disposed therein;
• and
• a computer connected to said first and second valves for automated control of said apparatus;
• wherein flow paths through said first and second columns are separated from one another and flow through said first and second columns may be effected simultaneously.