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AssigneeThe General Hospital Corporation
InventorsMehmet Toner, George Truskey, Ravi Kapur
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The invention features methods for separating cells from a sample (e.g., separating fetal red blood cells from maternal method begins with the introduction of a sample including cells into one or more microfluidic channels. In one embodiment, the device includes at least two processing steps. For example, a mixture of cells is introduced into a microfluidic channel that selectively allows the passage of a desired type of cell, and the population of cells enriched in the desired type is then introduced into a second microfluidic channel that allows the passage of the desired cell to produce a population of cells further enriched in the desired type. The selection of cells is based on a property of the cells in the mixture, for example, size, shape, deformability, surface characteristics (e.g., cell surface receptors or antigens and membrane permeability), or intracellular properties (e.g., expression of a particular enzyme).
A method for producing a population enriched in a first type of cell from a mixture of at least three types of cells, said method comprising the steps of:
A microfluidic system for the separation of one or more desired cells from a sample, said system comprising:
A microfluidic system for the separation of one or more desired cells from a sample, said system comprising:
A microfluidic system for the separation of a desired cell from a sample, said system comprising:
A microfluidic system for the separation of one or more desired cells from a sample, said system comprising:
A method for separating one or more cells of a second type from a sample, said method comprising the steps of:
A method for separating one or more cells of a second type from a sample, said method comprising the steps of:
A method for producing a population of cells enriched in a second type of cell, said method comprising the steps of:
A method for producing a population of cells enriched in a second type of cell, said method comprising the steps of:
A method for separating one or more cells of a second type from a sample, said method comprising the steps of:
A method for separating one or more cells of a second type from a sample, said method comprising the steps of:
A method for separating one or more cells of a second type from a sample, said method comprising the steps of:
A method of producing a cell population enriched in a second cell type, said method comprising the steps of:
A kit comprising:
A method of producing a depleted cell population, said method comprising the steps of:
A microfluidic device comprising obstacles disposed in a microfluidic channel, each of which is coated with a binding moiety.
A microfluidic device comprising:
A microfluidic device comprising:
The microfluidic device of 63, wherein the obstacles in the first region are coated with an anti-CD71, an anti-CD36, an anti-GPA, or an anti-CD45 antibody, or a combination thereof.
The microfluidic device of 63, wherein the obstacles in the second region are coated with an anti-CD71, an anti-CD36, an anti-GPA, or an anti-CD45 antibody, or a combination thereof.
Dependencies are inferred from the claim text.