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AssigneeChih Shin biomedical Technology Co., Ltd.
InventorJia-Ming Chang
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A method and an apparatus for rapid cell separation without using density gradient and antibody but using a column packed with resin particles. The interactions between cell surfaces and resin particles resulting in the different retention time of different cells in the column contribute to the separation of the specific cells from the mixed cell population. Therefore, no antibodies and specific chemical reagents are used in cell separation maintain the physiological status of separated cells. This invention can also apply to clinical use for fast and massive separation of blood sub-population, for the remove of leukemia cells from normal leucocytes in vivo or in vitro, and particularly for drug screening through the interaction between drugs and specific and non-specific cells.
An apparatus for rapid separation of cells without using density gradient and antibody, comprising a column and packing material in the column, wherein the column is used for separating different populations of the cells.
A method using an apparatus with a column and packing material in the column, wherein the column is used for separating different populations of cells, for rapid separation of the cells without using density gradient and antibody, wherein a cell sample is diluted with a buffer solution to a cell suspension having a pre-determined concentration, the cell suspension is loaded on the apparatus and is eluted with a buffer solution at a constant flow rate, the cell suspension eluent is collected in a test tube in a manner that a collecting test tube is successively replaced at a time interval or after collecting a constant number of cell suspension drop, and finally, the collected cells is identified into respective cell population so as to analyze the quality of cells thus separated.
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