Method for producing retinal neurocyte from neural stem cell derived from iris tissue and retinal, neurocyte produced by the process
AssigneeJapan Science and Technolohy Agency
InventorMitsuko Kosaka
Life of this patent
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Abstract
A method for producing retinal nerve cells by inducing differentiation of iris pigmented epithelial cells into the retinal nerve cells. In a first method, iris pigmented epithelial cells derived from a mammal and embryo retinal stem cells derived from a bird are co-cultured. In a second method, iris pigmented epithelial cells of a bird or a mammal is isolated, and the iris pigmented epithelial- cells is subjected to adherent culturing. According to these methods, the retinal nerve cells can be produced by using iris pigmented epithelial cells collected from a patient per se. Therefore, there is a possibility that highly effective regenerative medical treatment can be realized.
Claims14
A method for producing retinal nerve cells, the method comprising the steps of:
- co-culturing embryonic retinal stem cells and iris pigmented epithelial cells;
- and
- inducing differentiation of the iris pigmented epithelial cells into the retinal nerve cells.
A method for producing retinal nerve cells, the method comprising the steps of:
- isolating iris pigmented epithelial cells from an eyeball;
- and
- performing adherent culturing of the iris pigmented epithelial cells with a serum-free culture medium so as to induce differentiation of the iris pigmented epithelial cells into the retinal nerve cells.
A method for producing retinal nerve cells, the method comprising the steps of:
- isolating iris pigmented epithelial cells from an eyeball;
- starting adherent culturing by implanting the iris pigmented epithelial cells in a culture medium containing FGF2 and/or FGF9;
- and
- after the step of starting the adherent culturing, inducing differentiation of the iris pigmented epithelial cells into the retinal nerve cells by performing the adherent culturing of the iris pigmented epithelial cells by using the culture medium to which CNTF is added and from which FGF2 and/or FGF9 is removed.
Dependencies are inferred from the claim text.