Engineered bone marrow
OwnerAlan Flake, Alexander Krupnickinventors
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Abstract
An engineered bone marrow composition, preferably comprising bone marrow cells, pulverized bone, and type 1 collagen, can be transplanted into the portal system of a patient. The engineered bone marrow provides a microenvironment, for engraftment of hematopoietic stem cells, that increases levels of chimerism while decreasing or eliminating failure of engraftment.
Claims14
1-8. (canceled)
The method according to claim 19, wherein said combining comprises combining about 1×106 to about 5×108 bone marrow cells, with about 50-500 mg of said pulverized bone or bone substitute, and about 100 μl to about 750 μl of said collagen substitute, wherein said collagen substitute is a neutralized type I collagen matrix having a collagen concentration of about 1.0 mg/ml to about 2.0 mg/ml.
The method according to claim 19, wherein said combining comprises combining about 5×106 to about 1×108 bone marrow cells with about 50 mg to about 500 mg of said pulverized bone or bone substitute and about 100 μl to about 750 μl of said neutralized type I collagen matrix, wherein said collagen concentration is about 1.0 mg/ml to about 2.0 mg/ml.
The method according to claim 19, wherein said combining comprises combining about 1×107 to about 6×107 bone marrow cells with about 50 to about 500 mg of said pulverized bone or bone substitute and about 100 μl to about 750 μl of said neutralized type I collagen matrix, wherein said collagen concentration is about 1.0 mg/ml to about 2.0 mg/ml.
The method according to claim 19, wherein said bone substitute is selected from the group consisting of a calcium phosphate, a bioactive glass, and a bioactive ceramic.
A method of implanting an engineered bone marrow, comprising:
- providing engineered bone marrow comprising (a) a heterogeneous multipotential population of bone marrow cells comprising undifferentiated hematopoietic stem cells such that said bone marrow cells are present in said engineered bone marrow in a distribution which corresponds to a naturally occurring distribution of cell populations in a bone marrow
- (b) a pulverized bone or a bone substitute, and (c) a type I collagen or a collagen substitute;
- and
- introducing said engineered bone marrow into a vascular site in the portal system of a mammal and thereby implanting said engineered bone marrow in the mammal.
The method according to claim 19, wherein said engineered bone marrow is implanted in a pocket of an intramesenteric portal site.
Dependencies are inferred from the claim text.