Fibrin material and method for producing and using the same

A method for delivering fibrin hydrogel to a surface comprising the steps of:

• providing a liquid solution of fibrinogen;
• providing a liquid solution of thrombin;
• providing a liquid solution of a calcium chelating agent;
• providing a spray unit in fluid communication with the fibrinogen solution, the thrombin solution, and the chelating agent, the spray unit being capable of atomizing the fibrinogen solution, the thrombin solution, and the chelating agent into an aerosol with at least one energy source of a liquid energy, a mechanical energy, a mechanical energy, and an electric energy;
• spraying the fibrinogen solution onto the surface with the spray unit;
• spraying the chelating agent onto the surface with the spray unit;
• spraying the thrombin solution onto the surface with the spray unit;
• and
• mixing the fibrinogen solution the chelating agent and the thrombin solution.

A method for using a fibrin material to treat a patient, the method

• comprising the steps of:
• providing a first fibrin material having a pore size from about 2 µm to about 10 µm;
• providing a second fibrin material having a pore size from about 0.1 µm to about 5 µm;
• and being capable of retaining 80-90% of water upon compression by a force of 1-14 psi;
• applying the first fibrin material to a surface;
• and
• applying the second fibrin material to the first fibrin material.

A kit for forming a fibrin hydrogel comprising

• a container of proteins comprising fibrinogen;
• a container of thrombin;
• a container of a diluent, wherein the diluent has the ability to chelate calcium;
• and a
• mixing and application apparatus.

A kit for forming a fibrin hydrogel comprising:

• a container of proteins comprising fibrinogen, wherein the protein concentration is more than about 30 mg/ml;
• a container of thrombin, wherein the thrombin concentration is between about 0.1 IU/ml and about 1000 IU/ml:
• a container of a phosphate buffer solution;
• and
• a mixing and application apparatus, wherein the apparatus is selected from the group consisting of a syringe and a catheter.

A multiple layer fibrin material for treating a patient comprising:

• a fibrin film layer;
• a therapeutic fibrin hydrogel layer, the fibrin hydrogel having a water content of at least 90% by weight of the fibrin hydrogel and whereby the fibrin hydrogel retains from about 80% to about 90% of the water upon compression by a force of about 156 G;
• and
• a fibrin glue layer attaching the fibrin film layer to the fibrin hydrogel layer.