Fibrin cell supports and methods of use thereof
AssigneeDFB Pharmaceuticals, Inc.
InventorVincent Ronfard
Life of this patent
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Abstract
The present invention relates to fibrin cell supports for cell cultures formed by the mixture of plasma proteins including fibrinogen and thrombin. The fibrin cell supports are preferably used for preparing a culture of cells such as keratinocytes, recovering the culture in the form of a reconstituted tissue, and transporting same. The reconstituted tissue is particularly suitable for use as a skin graft.
Claims42
A fibrin cell support for skin grafts, comprising thrombin and fibrinogen, wherein the concentration of thrombin is between about 0.5 U/ml and about 2.5 U/ml.
A method of preparing a fibrin cell support, comprising:
- a) mixing equivalent volumes of a first solution comprising fibrinogen and a second solution comprising calcic thrombin, wherein the concentration of calcic thrombin is between about 1.0 U/ml and about 1.5 U/ml;
- and
- b) distributing said mixture onto a surface, such that a fibrin cell support is formed on said surface.
A method of using a fibrin cell support, comprising:
- a) contacting one or more cells with a fibrin cell support to form a skin replacement tissue, said support comprising calcic thrombin and fibrinogen, wherein the concentration of calcic thrombin is between about 1.0 U/ml and about 1.5 U/ml;
- and
- b) recovering the skin replacement tissue;
- c) optionally transporting the skin replacement tissue;
- and
- d) applying the skin replacement tissue as a graft.
A method of using a skin replacement tissue, comprising:
- a) contacting one or more keratinocytes with a solution comprising fibrinogen and calcic thrombin, wherein the concentration of calcic thrombin is between about 1.0 U/ml and about 1.5 U/ml, to form a skin replacement tissue, and
- b) optionally transporting said skin replacement tissue to a patient in need thereof.
A method of storing a fibrin cell support, comprising:
- a) contacting a fibrin cell support with a cylindrical inner carrier;
- b) inserting said fibrin cell support and said inner carrier in a hollow outer carrier, said outer carrier having an inner diameter larger than the outer diameter of the inner carrier;
- c) filling the space formed between the fibrin cell support and the inner wall of the outer carrier with cell media;
- and sealing the outer carrier so as to maintain a sterile environment, thereby storing the fibrin cell support.
A method of storing a fibrin cell support, comprising:
- a) contacting a fibrin cell support with a carrier, said carrier comprising a first and a second sheet, said first and said second sheets operably linked to each other, such that said support contacts said first sheet;
- b) filling the space formed between the fibrin cell support and said second sheet with cell media;
- and contacting said first and said second sheet so as to maintain a sterile environment, thereby storing the fibrin cell support.
Dependencies are inferred from the claim text.