Self-assembling protein matrix prepared from natural extracellular matrices
OwnerHugo Pedrozo, Mark Shusterinventors
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Abstract
The present invention provides a method for preparing a biomaterial involving extracting Collagen Type I (Col I) protein from small intestine submucosa (SIS) under conditions that preserve the native helical configuration. The extract of the present method contains a substantial amount of the alpha helical form of Col I. Further the present method includes inducing the Col I protein to form a polymer material in solution or on a target surface.
Claims28
A method for preparing a biomaterial comprising:
- extracting collagen Type I protein from submucosal tissue using an acidic reagent selected from the group consisting of acetic acid, citric acid, or formic acid, in the absence of detergents or enzymes, to produce a solution of collagen fibers wherein greater than 75% of the fibers have an α-helical tertiary structure;
- inducing self assembly of the collagen fibers to form the biomaterial.
A method of assembling a collagen type-I matrix on a surface, said method comprising:
- applying a composition comprising collagen type I on a surface, wherein said collagen type I is prepared by extracting a naturally occurring extracellular matrix with acetic acid at a pH of about 2-4;
- contacting the collagen type I composition with a salt solution to induce formation of a collagen type-I matrix on the surface.
A kit for applying a collagen matrix on a surface in situ, said kit comprising
- a collagen solution, wherein said collagen is isolated from a natural extracellular matrix using steps comprising extracting the natural matrix with an acidic reagent, selected from the group consisting of acetic acid, citric acid, or formic acid, at a pH of about 2 to about 3.5 and a temperature of less than 20° C.;
- and
- a salt solution comprising a salt selected from the group consisting of ammonium sulfate solution, sodium phosphate solution, phosphate buffered saline (PBS) solution and NaCl solution.
Dependencies are inferred from the claim text.