Loading of cells with antigens by electroporation
AssigneeMaxCyte, Inc.
InventorsLinda Liu, Jonathan Weiss
Life of this patent
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Abstract
Methods for loading an antigen-presenting cell with one ore more antigens are disclosed. Methods for the treatment and prevention of a disease in a subject using an antigen-presenting cell that has been electroporated with a composition of one or more antigens. Composition of one or more antigens comprises one or more antigens of a hyperproliferative cell, a microorganism or a microorganism-infected cell are also disclosed. In addition, compositions of antigen-presenting cells that have been loaded with one or more antigens of a hyperproliferative cell, a microorganism-infected cell or a microorganism using electroporation are disclosed.
Claims45
A method for loading an antigen-presenting cell with one or more antigens, comprising:
- a) preparing a mixture comprising antigen-presenting cells and a lysate having one or more antigens of a hyperproliferative cell, a microorganism-infected cell or a microorganism, wherein the number of antigen-presenting cells is greater than the number of cells represented in the lysate;
- and
- b) electroporating the mixture in a manner sufficient to load the one or more antigens into the antigen-presenting cells.
A method of treating or preventing a disease in a subject, comprising:
- a) loading an antigen-presenting cell with a lysate having one or more antigens of a hyperproliferative cell, a microorganism, or a microorganism-infected cell using electroporation;
- b) preparing a composition of said antigen-presenting cell;
- and
- c) administering to a subject in need thereof an effective amount of said composition.
Dependencies are inferred from the claim text.