Measurement of total reactive isocyanate groups in samples using bifunctional nucleophiles such as 1,8-diaminonaphthalene (dan)

A method for detecting and/or measuring total isocyanate in a sample comprising

• a) contacting a bifunctional nucleophilic, fused aromatic ring isocyanate derivatizing agent, wherein the two functionalities are amino functionalities in a symmetrical, planar relation to the molecular backbone so as to be capable of forming a cyclic reaction product and capable of reacting with an isocyanate group to form a urea, with a sample under conditions suitable for the formation of the cyclic reaction product capable of detection
• wherein the cyclic reaction product's structure is independent of that of the isocyanate group, and
• (b) detecting the presence or absence of the cyclic reaction product as an indication of the presence or absence of isocyanate in the sample.

A method for detecting and/or measuring total isocyanate in a sample comprising

• a) contacting 1,8-diaminonaphthalene with a sample containing isocyanate groups under conditions suitable for the formation of a reaction product capable of detection, and
• b) detecting the presence or absence of the reaction product as an indication of the presence or absence of isocyanate in the sample.

A method for determining the species of isocyanate in a sample comprising

• a) contacting a bifunctional nucleophilic isocyanate derivatizing agent with a sample, containing or suspected of containing isocyanate, under conditions suitable for the formation of an intermediate capable of detection, and
• b) detecting the presence or absence of the intermediate as an indication of the presence or absence of isocyanate species in the sample

A kit for detecting and/or measuring total isocyanate in a sample comprising

• a) a bifunctional nucleophilic derivatizing agent.

A filter for collecting a sample for detecting and/or measuring total isocyanate in a sample comprising

• a) an air sample collection filter, and
• b) a bifunctional nucleophilic derivatizing agent.

A kit for solid phase extraction (SPE) for detecting and/or measuring total isocyanate in a sample comprising

• a) a SPE cartridge, and
• b) a bifunctional nucleophilic derivatizing agent.