Viruses and virus-like particles for multiple antigen and target display
OwnerF. C. Allnutt, David Kyleinventors
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Abstract
The present invention relates to the display of antigenic or allergenic components along with a tissue-targeting component on viruses or virus-like particles. Capsid protein genes are recombinantly modified to contain the specified components then expressed within a host organism, such as yeasts, bacteria, or algae, and allowed to spontaneously form active virus particles or virus-like particles. The recombinant complexes (virus or virus-like particle) can then be purified or used in situ as a therapeutic tool for disease or allergy prevention. The expression of multivalent and multifunctional components to increase the immunogenicity of the recombinant complexes, especially on oral administration, is provided.
Claims48
A method of producing a recombinant virus comprising:
- (a) providing a viral genome;
- (b) inserting one or more first exogenous sequences encoding a desired protein or peptide into the genome;
- (c) inserting one or more second exogenous targeting sequences encoding a targeting element into the genome which has the function of targeting the complex to a specific location;
- and
- (d) transfecting an appropriate host, and allowing the host to produce the virus.
A genetic construct comprising a viral genome with a first exogenous sequence for display of a peptide or protein on a viral capsid protein, and a second exogenous sequence for display of a targeting moiety.
A method of producing a recombinant virus-like particle comprising:
- (a) providing a viral genome;
- (b) isolating at least one viral coat protein sequence;
- (c) inserting at least one first exogenous sequence encoding a protein or peptide of interest into the coat protein sequences;
- (d) inserting at least one second exogenous sequence encoding a targeting sequence;
- (e) cloning the viral coat protein sequence comprising the first and second exogenous sequences into an appropriate vector;
- and
- (f) transforming an appropriate host.
A genetic construct comprising at least one viral coat protein containing exogenous sequence for displayed peptides or proteins.
The method of claims 41-46, wherein the vaccine is used as a treatment for allergy.
Dependencies are inferred from the claim text.