Method of nucleic acid typing for selecting registered donors for cross-matching to transfusion recipients

A method of selecting a transfusion donor by determination of compatibility to a recipient by comparing combinations of polymorphic markers in a set of such markers, of candidate donors and a recipient:

• where said determination is performed following subjecting target nucleic acid samples amplified, respectively, from candidate donors and recipient genomic material, to a hybridization assay or to a capture-mediated elongation assay, or both, wherein an assay signal is generated by the individual hybridization or elongation events, as applicable, by a pair of probes wherein pair members are complementary, in whole or in part, to the subsequences of the amplified products which represent (and/or which are complementary to) polymorphic markers in the set, and an assay signal intensity pattern is generated from hybridization or elongation events such that each marker in the set is represented by a discrete assay signal intensity, the method comprising:
• determining the assay signal intensity pattern generated by said hybridization or elongation assay and forming, for each probe pair, a combination of signal intensities associated with the members of the pair;
• generating, from said combinations of signal intensities, a series of values (each value in the series selected from one of three possible values, respectively indicating a state of homozygous-normal, heterozygous or homozygous-variant) to thereby form a ternary reaction pattern;
• determining the polymorphic marker combinations represented by said ternary reaction pattern;
• and
• selecting from the candidate donors a donor having said polymorphic marker combination identical to that of the recipient.