Phosphoprotein affinity resins and methods for making and using the same
AssigneeClontech Laboratories, Inc.
InventorsGrigoriy Tchaga, Rajinder Bhatia
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Abstract
Phosphorylated protein (i.e., phosphoprotein) affinity resins and methods for making and using the same are provided. The subject resins include a substrate bonded to aspartate-based tetradentate ligand/metal ion complexes, where the tetradentate ligand/metal ion complexes have high specificity for phosphorylated amino acids. The subject resins find use in a variety of different applications, including phosphoprotein enrichment applications. Also provided are kits and systems that include the subject resins.
Claims26
A method of making a phosphoprotein affinity resin, said method comprising:
- (a) contacting an uncharged aspartate-based resin with a metal ion source comprising metal ions having selective affinity for phosphorylated amino acids to produce a metal ion charged aspartate-based resin, wherein said contacting occurs in the presence of acetic acid;
- and
- (b) removing non-complexed metal ion from said charged aspartate-based resin to produce said phosphoprotein affinity resin.
A phosphoprotein affinity resin comprising:
- a substrate bonded to aspartate-based tetradentate ligand/metal ion complexes, wherein substantially all of said complexes comprise a metal ion complexed with four ligands of a tetradentate ligand.
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Dependencies are inferred from the claim text.