Purified LH
AssigneeApplied Research Systems ARS Holding N.V.
InventorsGianfranco Paradisi, Mara Rossi, Laura Scaglia
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Abstract
Recombinant human luteinizing hormone (LH) having a specific bioactivity of from 20,522 to 31,229 IU/mg and obtainable from a process for the purification of recombinant LH from a sample of crude recombinant LH in the supernatant of CHO cells which comprises the combined use of ion-exchange chromatography and reverse phase HPLC. The ion-exchange chromatography and the reverse phase HPLC are performed twice and the final use of a gel permeation column allows the purification from any residual traces of contaminants. The recombinant LH can be formulated into a pharmaceutical composition and used for treating a fertility disorder.
Claims10
The recombinant LH of claim 10 having a specific bioactivity of from 20,522 to 31,229 IU/mg and obtainable by a process which comprises:
- subjecting said sample to ion-exchange chromatography with an anion exchange resin;
- subjecting said sample to reverse phase HPLC;
- and
- isolating the recombinant LH.
The recombinant LH of claim 10, having a specific bioactivity of about 25,000 IU/mg.
A pharmaceutical composition, comprising a therapeutically effective amount of the recombinant LH of claim 10 and suitable excipients thereof.
A recombinant LH having a specific bioactivity of from 20,522 to 31,229 IU/mg.
Dependencies are inferred from the claim text.