CaMKII/calcium channel binding-related compositions and methods
OwnerGeoffrey Pitt, Andy Hudmon +1inventors
InventorsGeoffrey Pitt, Andy Hudmon, Howard Schulman
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Abstract
This invention provides a method for determining whether an agent inhibits binding between CaMKII and a Ca+2 channel. Furthermore, this invention provides a method for inhibiting binding between CaMKII and a Ca+2 channel in a cell. Finally, this invention provides methods of treating cardiac arrhythmia and neurological disorders.
Claims20
A method for determining whether an agent inhibits binding between CaMKII and a calcium channel, comprising:
- (a) contacting (i) CaMKII, (ii) the calcium channel or a fragment thereof comprising a portion whose amino acid sequence is TVGKF(Y/I) and (iii) the agent, under conditions which, in the absence of the agent, permit binding between CaMKII and the channel or fragment thereof;
- (b) determining the amount of binding between CaMKII and the channel or fragment thereof in step (a);
- and
- (c) comparing the amount of binding determined in step (b) with the amount of binding between CaMKII and the channel or fragment thereof in the absence of the agent, whereby a lower amount of binding in the presence of the agent indicates that the agent inhibits binding between CaMKII and the calcium channel.
15-25. (canceled)
A method for inhibiting binding between CaMKII and a calcium channel in a cell comprising contacting the cell with an agent that inhibits binding between CaMKII and an L-Type and/or P/Q-Type calcium channel.
A method for inhibiting binding between CaMKII and a calcium channel in a cell comprising contacting the cell with a polypeptide comprising a fragment of an L-Type calcium channel comprising a portion whose amino acid sequence is TVGKF(Y/I).
30-42. (canceled)
Dependencies are inferred from the claim text.