US 2006/0078993 A1 · Isolation, cultivation and uses of stem/progenitor cells

Abstract

The present invention relates to a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and/or mesenchymal stem/progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem/progenitor cells into epithelial and/or mesenchymal cells.

Claims36

Dependent claims

A method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, the method comprising:

(a) separating the amniotic membrane from the other components of the umbilical cord in vitro;
(b) culturing the amniotic membrane tissue obtained in step (a) under conditions allowing cell proliferation;
and
(c) isolating the stem/progenitor cells.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

11/205,248Filed 15 Aug 2005

Priority

WO PCT/SG05/001743 Jun 2005

Published

13 Apr 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification31 codes

A61K 35/28
Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically…:…
A61P 1/18
Drugs for disorders of the alimentary tract or the digestive system: for pancreatic disorders, e.g. pancreatic enzymes
A61K 9/0014
Galenical forms characterised by the site of application: skin, i.e. galenical aspects of topical compositions
A61K 9/06
Medicinal preparations characterised by special physical form: ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels

Isolation, cultivation and uses of stem/progenitor cells

Life of this patent

Isolation, cultivation and uses of stem/progenitor cells

Life of this patent

Abstract

Claims36