Novel vectors for animal cells and use thereof
AssigneeKirin Beer Kubushiki Kaisha
InventorsElisa Misawa, Hiroaki Yajima, Keiji Kondo
Life of this patent
Estimating the term…
Abstract
Protein synthesis inhibitor resistance genes (typified by a cycloheximide resistance gene) are capable of imparting resistance to a protein synthesis inhibitor (typified by cycloheximide) to animal cells sensitive to the inhibitor. The genes have a sequence mutated by substitution in a gene encoding a ribosome-constituting protein derived from an animal. The genes may be placed in recombinant vectors, including expression vectors containing the gene together with a foreign protein structural gene.
Claims6
A process for selecting an animal cell strain resistant to a protein synthesis inhibitor, characterized by introducing an expression vector containing a protein synthesis inhibitor resistance gene which is capable of imparting the resistance to a protein synthesis inhibitor to animal cells sensitive to the inhibitor and has a sequence mutated by substitution with a gene encoding a ribosome-constituting protein derived from an animal, and a foreign protein structural gene in a state to be expressed into animal cells sensitive to the protein synthesis inhibitor, culturing the cells and then selecting the target cell strain by using the protein synthesis inhibitor resistance gene as a selection marker.
Dependencies are inferred from the claim text.