US 2006/0078985 A1 · Device and method for quantitatively measuring immobilized sample

Abstract

Evaluation of an amount of immobilized sample in a surface plasmon resonance (SPR) immunoassay is provided. To immobilize the sample, a sensor unit has a sensing surface. The sensor unit includes a transparent dielectric medium, and a metal film having the sensing surface. The metal film is connected with the dielectric medium for constituting a metal/dielectric interface. The sensing surface is adapted to sensing reaction of the sample. Then illuminating light reflected by the metal/dielectric interface is received with a photo detector such as a CCD upon applying the illuminating light to the metal/dielectric interface, to detect attenuation of the illuminating light and output a detection signal thereof. The evaluated amount of the sample immobilized on the sensing surface is evaluated according to the detection signal. Before immobilization, the sensor unit is tested for the photo detector to output an initial detection signal, which is considered for the evaluation.

Claims23

Dependent claims

An immobilized sample measuring device comprising:

an immobilizing stage for immobilization of a sample on a sensing surface of at least one sensor unit, wherein said sensor unit includes a transparent dielectric medium, and a thin film, having a first surface and said sensing surface back to said first surface, said first surface being connected with said dielectric medium for constituting a thin film/dielectric interface, said sensing surface being adapted to sensing reaction of said sample immobilized thereon;
a signal detecting stage, loaded with said sensor unit after said sample immobilization in said immobilizing stage, having a photo detector for receiving illuminating light reflected by said thin film/dielectric interface upon applying said illuminating light to said thin film/dielectric interface in a form conditioned for total reflection, to detect attenuation of said illuminating light and output a detection signal thereof;
and
an arithmetic processor for quantitatively evaluating an amount of said sample immobilized on said sensing surface according to said detection signal.

1 more element

An immobilized sample measuring method comprising:

an immobilizing step of immobilizing a sample on a sensing surface of at least one sensor unit, wherein said sensor unit includes a transparent dielectric medium, and a thin film, having a first surface and said sensing surface back to said first surface, said first surface being connected with said dielectric medium for constituting a thin film/dielectric interface, said sensing surface being adapted to sensing reaction of said sample immobilized thereon;
a signal detecting step of receiving illuminating light reflected by said thin film/dielectric interface with a photo detector upon applying said illuminating light to said thin film/dielectric interface in a form conditioned for total reflection, to detect attenuation of said illuminating light and output a detection signal thereof;
and
an arithmetic processing step of quantitatively evaluating an amount of said sample immobilized on said sensing surface according to said detection signal.

1 more element

Dependencies are inferred from the claim text.

Device and method for quantitatively measuring immobilized sample

Life of this patent

Device and method for quantitatively measuring immobilized sample

Life of this patent

Abstract

Claims23