Method for identifying antimicrobial molecules which interfere with apolipoprotein N-acyltransferase activity
OwnerCarine Robichon, Anthony Pugsley +1inventors
InventorsCarine Robichon, Anthony Pugsley, Dominique Vidal-Ingigliardi
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Abstract
Products, compositions and methods useful for screening compounds or test molecules for their ability to modulate apolipoprotein N-acyltransferase activity.
Claims33
An isolated or purified polypeptide comprising one Lnt periplasmic segment selected from the group consisting of sequences SEQ ID NO:
- 3, 4 and 5, sequences which consist essentially of amino acids residues 28-33, 76-87 or 212-488 of SEQ ID NO:
- 2, or a fragment thereof.
A method for identifying a compound which modulates the activity of Lnt protein comprising contacting a compound with a cell expressing a polypeptide comprising at least one periplasmic segment of the Lnt protein, and determining the amount of apolipoprotein which accumulates in the inner membrane and/or the amount of lipoprotein in the outer membrane and/or associated with the peptidoglycan.
A method for identifying a compound which modulates the activity of Lnt protein comprising:
- contacting a cell expressing a polypeptide comprising one periplasmic segment of the Lnt protein with a test compound and determining membrane integrity or cell viability compared to a cell not contacted with said test compound.
An isolated or purified polynucleotide encoding one or more Lnt protein domains described by FIG. 9 or by FIG. 10;
- the complement thereof;
- or a fragment thereof.
Dependencies are inferred from the claim text.