Novel stationary phases for use in high-performance liquid chromatography

A substrate for peptide synthesis comprising a silica substrate, and a silane arrange in the form:

• Wherein R′ is selected from the group consisting of vinyl, allyl, ethynyl, and propynyl, R″ is selected from the groups consisting of methyl, ethyl, propyl, isopropyl, vinyl, allyl, ethynyl, and propynyl, R is —(CH2)3—NH2 and the O moiety is covalently attached to the silica substrate.

A method for the chromatographic separation comprising:

• applying a sample to a stationary phase, said stationary phase comprising a stable support structure comprising a substrate and a monofunctional silane, at least one of monofunctional silane having at least one unsaturated hydrocarbon group, R′ and an additional functional group, R, and wherein the silane structure is of form:
• Where R′ is independently selected from the group consisting of alkenyl, alkynyl, and phenyl, R″ is selected from the group consisting of alkyl, substitute alkyl, alkenyl, substitute alkenyl, alkynyl, and aryl, substitute aryl, R is selected from the group consisting of alkyl, substitute alkyl, alkenyl, substitute alkenyl, alkynyl, and aryl, substitute aryl, alkylamine, amide, ether, alcohol, carbamate, ester, an anion exchanger, and a cation exchange.

A column for use in chromatographic separations comprising:

• a substrate comprising a monofunctional silane, having at least one unsaturated hydrocarbon group, R′, covalently attached to the substrate, containing the silane structure is of form:
• Where R′ is independently selected from the group consisting of alkenyl, alkynyl, and phenyl, R″ is selected from the group consisting of alkyl, substitute alkyl, alkenyl, substitute alkenyl, alkynyl, and aryl, substitute aryl, R is selected from the group consisting of alkyl, substitute alkyl, alkenyl, substitute alkenyl, alkynyl, and aryl, substitute aryl, alkylamine, amide, ether, alcohol, carbamate, ester, an anion exchanger, and a cation exchanger.

A process for the manufacture of substrate for chromatography, comprising:

• (i) preparing divinyl alkyl silanes and monovinyl alkyl silanes for bonding,
• (ii) bonding a silica substrate with the divinyl alkyl silane, or monovinyl alkyl silane, or a mixture of two or three silanes with at least one silane having monovinyl alkyl silane to produce a bonded phase,
• (iii) bonding at least a fraction of residual unbonded silica surface with a silane selected form the group consisting of monovinyl, divinyl, and trivinyl silane.