Measuring device and measuring method for detecting analytes

A method for the production of a device (10) for the detection and determination of the concentration of at least one analyte dissolved in a liquid medium, wherein

• a) an electrode material is worked to a paste and this paste is applied to a carrier (20) in such a way that a multiple electrode set consisting of at least one working electrode (40) and at least one reference electrode (30) and adjoining conductive tracks (42) and contacts (44) is formed,
• b) said carrier (20) provided with said multiple electrode set is dried at elevated temperature,
• c) ligands and/or biologically active specific binding molecules and/or catalytically active proteins are applied to said carrier (20), namely in said region of said multiple electrode set or in a region which is disposed upstream of said multiple electrode set and
• d) an elongated support (52), a cover (54), and a liquid absorption element (60) are fixed to the carrier (20) thus obtained such that said cover (54) and said carrier (20) are spaced from one another.

A method for the detection and determination of the concentration of at least one analyte dissolved in a liquid medium using a device (10) which consists of at least one working electrode (40) and at least one reference electrode (30, wherein

• a) a definable amount of said liquid medium is applied to a sample application zone (22) of said device (10),
• b) a specified period of time is allowed to elapse in order to allow interaction of said analyte(s) with ligands and/or biologically active specific binding molecules and/or catalytically active proteins to take place,
• c) a solution is applied to the sample application zone (22), which contains at least one reaction-specific substrate for a catalytically active protein or a number of catalytically active proteins,
• d) said device (10) is connected to an electrical measuring apparatus, and
• e) after an incubation time, an electrical signal is read off on said measuring apparatus connected to said device (10) for each analyte to be determined and a corresponding concentration of the respective analyte is assigned to this signal with the aid of a calibration curve.