Method for reducing allergens in an enclosure

A method for reducing allergens in an enclosure, wherein the allergens are selected from the group consisting of airborne particles, airborne fungal spores and surface fungal spores, and wherein air in the enclosure is in communication with an air handling system, comprising the steps of:

• a) applying vacuum suction to the air handling system;
• b) applying to the air handling system an aqueous solution comprising a first antimicrobial agent wherein the solution is applied using forced air;
• c) applying vacuum suction to the carpets and upholstery in the enclosure with a vacuum cleaner fitted with a High-Efficiency Particulate Air (HEPA) filter;
• d) applying a formulation comprising a cleansing agent to the carpets and upholstery in the enclosure, wherein the cleansing agent is a composition comprising a non-ionic surfactant, benzlkonium chloride, 2-propanol, or Di-N-alkyl (C8-C10)—N,N-dimethylammonium chloride;
• e) fogging surfaces in the enclosure with a formulation comprising a second antimicrobial agent, wherein the second antimicrobial agent is a composition comprising an aqueous organosilane, a quaternary chloride or a hydantoin resin;
• f) releasing ozone within the enclosure at a rate of at least 2 parts per million (ppm) for at least one hour;
• g) terminating the release of ozone and allowing the ozone concentration in the enclosure to return to below 0.05 ppm;
• h) placing a reservoir containing a composition comprising tea tree oil into the air handling system, such that when the air handling system is operated, the tea tree oil is dispersed into the enclosure;
• wherein the tea tree oil can dissipate such that air that is drawn into the air handling unit comes into contact with the dissipating tea tee oil and disperses the tea tee oil into the enclosure;
• i) initiating operation of the air handling system;
• and
• j) initiating operation of an air purifier, which air purifier comprises a (HEPA) filter and an active carbon filter;
• wherein, after initiating operation of the air purifier of step j, and for a period of at least 90 days, the airborne particles in the enclosure are fewer than 1.0 million airborne particles per cubic meter of air;
• and
• the airborne fungal spores in the enclosure are at present in an amount at least 1.1 times below the amount of airborne fungal spores in the air outside the enclosure before performing steps a) through j);
• and,
• the amount of surface fungal spores present in the enclosure is at least 3.0 times below the amount of fungal spores in the enclosure before performing steps a) through j).