Corn event DAS-59122-7 and methods for detection thereof

A DNA detection kit specific for junction DNA of maize event DAS-59122-7 and its progeny comprising at least one DNA molecule of a sufficient length of contiguous DNA polynucleotides to function in a DNA detection method, that is homologous or complementary to a sequence selected from the group consisting of:

• a) the nucleotide sequence set forth in SEQ ID NO:
• 21;
• and
• b) the nucleotide sequence set forth in SEQ ID NO:
• 22.

A kit for identifying event DAS-59122-7 in a biological sample, said kit comprising a specific probe comprising a sequence which hybridizes with sequences selected from the group consisting of:

• a) the sequences of SEQ ID NO:
• 19 and SEQ ID NO:24;
• and
• b) the sequences of SEQ ID NO:
• 20 and SEQ ID NO:
• 24;
• contiguous therewith.

A DNA construct comprising:

• a first, second, and third expression cassette, wherein said first expression cassette in operable linkage comprises:
• (a) a maize ubiquitin promoter;
• (b) a 5′ untranslated exon of a maize ubiquitin gene;
• (c) a maize ubiquitin first intron;
• (d) a Cry34Ab1 encoding DNA molecule;
• and
• (e) a PinII transcriptional terminator;
• said second expression cassette comprising in operable linkage
• (i) a wheat peroxidase promoter;
• (ii) a Cry35Ab1 encoding DNA molecule;
• and
• (iii) a PinII transcriptional terminator;
• and said third expression cassette comprising in operable linkage
• (1) a CaMV 35S promoter;
• (2) a pat encoding DNA molecule;
• and
• (3) a 3′ transcriptional terminator from (CaMV) 35S.

A method of detecting the presence of maize event DAS-59122-7 or progeny thereof in a biological sample, comprising:

• (a) extracting a DNA sample from said biological sample;
• (b) providing a pair of DNA primer molecules selected from the group consisting of:
• i) the sequences of SEQ ID NO:
• 18 and SEQ ID NO:
• 1;
• ii) the sequences of SEQ ID NO:10 and SEQ ID NO:9;
• iii) the sequences of SEQ ID NO:2 and SEQ ID NO:17;
• and
• iv) the sequences of SEQ ID NO:8 and SEQ ID NO:17;
• (c) providing DNA amplification reaction conditions;
• (d) performing said DNA amplification reaction, thereby producing a DNA amplicon molecule;
• and
• (e) detecting said DNA amplicon molecule, wherein the detection of said DNA amplicon molecule in said DNA amplification reaction indicates the presence of maize event DAS-59122-7.

A method of detecting the presence of DNA corresponding to the DAS-59122-7 event in a sample, the method comprising:

• (a) contacting the sample comprising maize DNA with a polynucleotide probe that hybridizes under stringent hybridization conditions with DNA from maize event DAS-59122-7 and does not hybridize under said stringent hybridization conditions with a non-DAS-59122-7 maize plant DNA;
• (b) subjecting the sample and probe to stringent hybridization conditions;
• and
• (c) detecting hybridization of the probe to the DNA, wherein detection of hybridization indicates the presence of the DAS-59122-7 event.

A pair of DNA molecules comprising:

• a first DNA molecule and a second DNA molecule, wherein the DNA molecules are of a sufficient length of contiguous nucleotides of a sequence selected from the group consisting of:
• a) the sequence set forth in SEQ ID NO:
• 21 or its complement;
• and
• b) the sequence set forth in SEQ ID NO:
• 22 or its complement;
• to function as DNA primers or probes diagnostic for DNA extracted from a DAS-59122-7 corn plant or progeny thereof.

A method of detecting the presence of the DAS-59122-7 event insertion in corn tissue comprising:

• (a) selecting a primer pair each comprising at least ten nucleotides from SEQ ID NO:21 or SEQ ID NO:
• 22 wherein each member of the pair is on opposite sides of a sequence diagnostic for said DAS-59122-7 event insertion;
• (b) contacting a sample of said corn tissue with said primer pair;
• (c) performing DNA amplification and analyzing for an amplicons.

A method of detecting the presence of the DAS-59122-7 event insertion in corn tissue comprising:

• (a) contacting a sample of said corn tissue with a polynucleotide probe that hybridizes under stringent hybridization conditions with one or more DNA sequence selected from the group consisting of SEQ ID NO:
• 32, 33, 34, and 35 and complements thereof;
• (b) subjecting said sample and probe to stringent hybridization conditions;
• and
• (c) analyzing for hybridization of the probe.