Plant-specific genetic elements and transfer cassettes for plant transformation
AssigneeJ.R. Simplot Company
InventorsCaius Rommens, Oleg Bougri, Hua Yan
Life of this patent
Estimating the term…
Abstract
The present invention provides nucleic acid molecules and sequences, particularly those identified and obtained from plants, that are useful for transferring and integrating one polynucleotide into another via plant transformation techniques.
Claims29
A cassette, comprising (1) a first polynucleotide, comprising a sequence that is (i) nicked when exposed to an enzyme involved in bacterial-mediated plant transformation and (ii) not identical to a bacterial border sequence;
- (2) a second polynucleotide, which may be (i) an imperfect or perfect repeat of the first polynucleotide, or (ii) a bacterial T-DNA border;
- (3) a desired polynucleotide positioned between the first and second polynucleotides;
- and (4) at least one of (a) UI region, (b) DI region, (c) UF region, or (d) AF region.
A plant transformation cassette, comprising at least one of (1) a polynucleotide comprising a sequence depicted in any one of the group consisting of SEQ ID NOs:
- 8, 9, 11-13, 15-17, 28-50, 85, and 86, wherein the DNA sequence comprising the 3′-end of the polynucleotide and DNA downstream thereof comprises the sequence of SEQ ID NO:
- 122;
- (2) a polynucleotide comprising (i) a sequence depicted in any one of the group consisting of SEQ ID NOs:
- 8, 9, 11-13, 15-17, 28-37, 38-51, 85-86, 189, 190, 194-196, and 198, and (ii) a DNA sequence positioned downstream of the sequence of (i), wherein the sequences of (i) and (ii) together comprise a cytosine cluster that comprises a sequence that shares at least 70% sequence identity with any one of SEQ ID NOs:
- 187-188 and 215-218;
- and (3) a polynucleotide comprising a sequence depicted in any one of the group consisting of SEQ ID NOs:
- 8, 9, 11-13, 15-17, 28-37, 38-51, 85-86, 189, 190, 194-196, and 198, wherein the 5′-end of the polynucleotide abuts a pyrimidine-rich element that shares at least 70% sequence identity with any one of SEQ ID NOs:
- 21 and 199-208.
A cassette, comprising (1) a first polynucleotide, comprising a sequence that is nicked when exposed to an enzyme involved in bacterium-mediated plant transformation;
- (2) a second polynucleotide that has greater than 70% sequence identity to at least 15 nucleotides of any one of SEQ ID NOs:
- 133-137 or a functional homolog or derivative thereof;
- and (3) a desired polynucleotide positioned between the first and second polynucleotides.
A method for transforming a plant, comprising (1) transforming a plant with a transformation plasmid that is suitable for bacterium-mediated plant transformation, wherein the plasmid comprises a transfer-DNA that is delineated by (i) left and right transfer-DNA border sequences, and which comprises (ii) a non-autonomous transposable element, which comprises a desired polynucleotide, and a (iii) a transposase gene, wherein the non-autonomous transposable element and the transposase gene are positioned between the left and right border sequences, and (2) selecting a plant that stably comprises in its genome the non-autonomous transposable element but not the transfer-DNA.
A plant transformation plasmid, comprising a cassette, which comprises (1) a first polynucleotide, comprising a sequence that is (i) nicked when exposed to an enzyme involved in bacterium-mediated plant transformation and (ii) not identical to a bacterial border sequence;
- (2) a second polynucleotide, which may be (i) an imperfect or perfect repeat of the first polynucleotide, or (ii) a bacterial T-DNA border;
- and (3) a region comprising a virC2 gene.
Dependencies are inferred from the claim text.