Survivin, a protein that inhibits cellular apoptosis, and its modulation
AssigneeYale University
InventorDario Altieri
Life of this patent
Estimating the term…
Abstract
The present invention provides the amino acid of a protein that inhibits cellular apoptosis, herein termed the Survivin protein and nucleic acid molecules that encode Survivin. Based on this disclosure, the present invention provides isolated Survivin protein, isolated Survivin encoding nucleic acid molecules, methods of isolating other members of the Survivin family of proteins, methods for identifying agents that block Survivin mediated inhibition of cellular apoptosis, methods of using agents that block Survivin mediated inhibition or Survivin expression to modulate biological and pathological processes, and methods of assaying Survivin activity.
Claims23
1-59. (canceled)
An oligonucleotide that hybridizes under stringent conditions with a polynucleotide having the sequence SEQ ID NO:
- 35, but does not hybridize under stringent conditions with a polynucleotide having the sequence of human EPR-1 mRNA, wherein the stringent conditions are selected from the group consisting of:
- (1) washing with 0.015 M sodium chloride, 0.0015 M sodium titrate, 0.1% SDS at 50° C.;
- (2) hybridization in 50% (vol/vol) formamide with 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42° C.;
- and (3) hybridization in 50% formamide, 5×SSC (0.75 M sodium chloride, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 0.1% SDS, or the complement of said oligonucleotide.
An oligonucleotide that hybridizes under stringent conditions with a polynucleotide having the sequence of the coding sequence of survivin, but does not hybridize under stringent conditions with a polynucleotide having the sequence of the coding sequence of human EPR-1, wherein the stringent conditions are selected from the group consisting of:
- (1) washing with 0.015 M sodium chloride, 0.0015 M sodium titrate, 0.1% SDS at 50° C.;
- (2) hybridization in 50% (vol/vol) formamide with 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42° C.;
- and (3) hybridization in 50% formamide, 5×SSC (0.75 M sodium chloride, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 0.1% SDS, or the complement of said oligonucleotide.
An oligonucleotide that hybridizes under stringent conditions with the mRNA encoding human survivin, but does not hybridize under stringent conditions with the mRNA encoding human EPR-1, wherein the stringent conditions are selected from the group consisting of:
- (1) washing with 0.015 M sodium chloride, 0.0015 M sodium titrate, 0.1% SDS at 50° C.;
- (2) hybridization in 50% (vol/vol) formamide with 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42° C.;
- and (3) hybridization in 50% formamide, 5×SSC (0.75 M sodium chloride, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC and 0.1% SDS, or the complement of said oligonucleotide.
Dependencies are inferred from the claim text.