Process of cell electrofusion
OwnerJustin Tessie, Jacques Bartholeynsinventors
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Abstract
The invention relates to the use of an electrical field applied to a mixture containing a first type of cell (C1), a second type of cell (C2), a bispecific ligand able to bind to C1 and/or to C2 and non-covalent complexes formed between C1, C2 and the bispecific ligand, for the preparation of a cell population enriched in C1-C2 heterohybrids or for the preparation of C1-C2 heterohybrids.
Claims39
(canceled)
A method for production of a cell population enriched in C1-C2 heterohybrids comprising a step of applying an electrical field to a mixture containing a first type of cell (C1), a second type of cell (C2), a bispecific ligand able to bind to C1 and/or to C2 and non-covalent complexes formed between C1, C2 and the bispecific ligand, said electrical field, being designed to induce cellular fusion, enabling formation of heterohybrids.
A cell population enriched in C1-C2 heterohybrids comprising a percentage of heterohybrids from about 15 to about 80% of cells.
A kit for the production of a cell population enriched in C1-C2 heterohybrids, said kit comprising fusion chamber, bags, connecting tubes and chambers in plastic and/or plastic and metal, media, washing solutions and a device allowing the flow of the mixture.
(canceled)
Dependencies are inferred from the claim text.