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US 2006/0068477 A1Published application
Methods for the production of products in host cells
OwnerTimothy Dodge, Fernando Valleinventors
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OwnerTimothy Dodge, Fernando Valleinventors
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The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.
A process for producing an ascorbic acid intermediate in a recombinant host cell comprising, culturing a host cell capable of producing said ascorbic acid intermediate in the presence of glucose under conditions suitable for the production of said ascorbic acid intermediate wherein said host cell comprises at least one deletion selected from the group of polynucleotides encoding kinases from the group consisting of glucokinase and gluconokinase.
A method of enhancing the production of an ascorbic acid intermediate from a carbon source comprising,
Dependencies are inferred from the claim text.