Coupled enzymatic reaction system using a formate dehydrogenase derived from candida boidinii
AssigneeDegussa AG
InventorsHarald Groger, Werner Hummel
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Abstract
The present process relates to a coupled enzymatic reaction system which is implemented in a two-phase system consisting of organic phase and aqueous phase. The system operates with cofactor-dependent enzymes, the cofactor being continually regenerated enzymatically by an FDH derived from C. boidinii.
Claims12
A coupled enzymatic reaction system comprising an NADH-dependent enzymatic transformation of an organic compound with an alcohol dehydrogenase and an enzymatic regeneration of the NADH with the formate dehydrogenase derived from Candida boidinii or mutants thereof in a two-phase solvent system, wherein an aqueous phase is in contact with a liquid organic phase.
Reaction system according to one or more of the preceding claims,
- characterised in that
- the organic compound is present prior to the start of the reaction in a concentration of >25 mM per L solvent mixture, in particular >100 mM per L solvent mixture.
Reaction system according to one or more of the preceding claims,
- characterised in that
- the system contains no surfactants.
Reaction system according to one or more of the preceding claims,
- characterised in that
- an alcohol dehydrogenase derived from Lactobacillus kefir is employed as enzyme for the transformation of the organic compound.
Dependencies are inferred from the claim text.