N-terminal and C-terminal markers in nascent proteins
AssigneeAmberGen, Inc.
InventorsKenneth Rothschild, Sadanand Gite, Jerzy Olejnik
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Abstract
This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Methods are described for incorporating N-terminal, C-terminal and (optionally) affinity markers into a nascent protein
Claims13
A composition, comprising a nascent protein bound to an antibody, wherein said nascent protein was produced in a translation system capable of incorporating an N-terminal marker and a C-terminal marker into said nascent protein.
A composition, comprising a nascent protein produced in a translation system capable of incorporating an N-terminal marker and a C-terminal marker into said nascent protein, and a ligand bound to said N-terminal marker, wherein said nascent protein is encoded by nucleic acid suspected of containing a mutation that causes chain truncation.
A composition, comprising a nascent protein produced in a translation system capable of incorporating an N-terminal marker and a C-terminal marker into said nascent protein, and a ligand bound to said C-terminal marker, wherein said nascent protein is encoded by nucleic acid suspected of containing a mutation that causes chain truncation.
A composition comprising a truncated nascent protein bound to a ligand, said truncated nascent protein comprising an N-terminal marker, said ligand bound to said N-terminal marker and attached to a well of a microtiter plate.
Dependencies are inferred from the claim text.