Fungal antigens and process for producing the same
AssigneeTakara Bio Inc.
InventorsKazutoh Takesako, Shigetoshi Mizutani, Masahiro Endo, Ikunoshin Kato
Life of this patent
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Abstract
There can be provided a fungal antigen which is an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed; a process for producing the same; a nucleic acid encoding the fungal antigen; a biologic product containing the fungal antigen; a method of stimulating immunological responses by using the biologic product; a method of suppressing allergic reaction to fungi in a vertebrate; and a method for diagnosing a disease caused by fungi in a vertebrate.
Claims20
An isolated nucleic acid encoding a fungal antigen which is an antigenic protein having a vaccine activity or an allergen activity originating from Candida albicans, wherein said antigenic protein comprises the amino acid sequence as shown by SEQ ID NO:5 in Sequence Listing.
An isolated nucleic acid encoding a fungal antigen which originates from the genus Candida and has a vaccine activity or an allergen activity, wherein said nucleic acid hybridizes to the complete complement of a nucleic acid of the nucleotide sequence as shown in SEQ ID NO:7 in Sequence Listing in 6×SSC containing 0.5% SDS, 0.1% bovine serum albumin (BSA), 0.1% polyvinyl pyrrolidone, 0.1% Ficoll 400, and 0.01% denatured salmon sperm DNA at 50° C.;
- followed by washing initially at 37° C. in 2×SSC containing 0.5% SDS and changing the SSC concentration to 0.1×SSC and the SSC temperature to 50° C.
An isolated nucleic acid encoding a fungal antigen which is an antigen comprising an antigenic protein having a vaccine activity or an allergen activity originating from Candida albicans, wherein said antigenic protein comprises the amino acid sequence as shown by SEQ ID NO:6 in Sequence Listing.
An isolated nucleic acid encoding a fungal antigen which originates from the genus Candida and has a vaccine activity or an allergen activity, wherein said nucleic acid hybridizes to the complete complement of a nucleic acid of the nucleotide sequence as shown in SEQ ID NO:8 in Sequence Listing in 6×SSC containing 0.5% SDS, 0.1% bovine serum albumin (BSA), 0.1% polyvinyl pyrrolidone, 0.1% Ficoll 400, and 0.01% denatured salmon sperm DNA at 50° C.;
- followed by washing initially at 37° C. in 2×SSC containing 0.5% SDS and changing the SSC concentration to 0.1×SSC and the SSC temperature to 50° C.
A process for producing a fungal antigen which is an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed, characterized in that said process comprises the steps of:
- (1) obtaining living fungal cells;
- (2) obtaining fungal cells of which cell wall has been substantially removed or at least partially removed;
- (3) bursting the fungal cells of which cell wall has been substantially removed or at least partially removed;
- and
- (4) obtaining an insoluble fraction.
A process for producing a fungal antigen which is a solubilized fraction extracted and separated from an insoluble fraction obtainable from fungal cells of which cell wall has been substantially removed or at least partially removed, characterized in that said process comprises the steps of:
- (1) obtaining living fungal cells;
- (2) obtaining fungal cells of which cell wall has been substantially removed or at least partially removed;
- (3) bursting the fungal cells of which cell wall has been substantially removed or at least partially removed;
- (4) obtaining an insoluble fraction;
- and
- (5) extracting and separating a solubilized fraction from the insoluble fraction.
Dependencies are inferred from the claim text.