Novel soluble CD14 antigen
AssigneeMochida Pharmaceutical Co., Ltd.
InventorsShoji Furusako, Kamon Shirakawa, Jiro Hirose
Life of this patent
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Abstract
There are provided a soluble CD14 antigen which is a novel in vivo protein useful as a marker for diagnosing sepsis and has the following characteristic features 1) to 3): 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions; 2) an amino acid sequence in which the amino acid sequence of SEQ ID NO:1 is present on its N terminal; and 3) ability to specifically bind to an antibody prepared by using a peptide comprising 16 amino acid residues described in SEQ ID NO:2 for the antigen; and a recombinant soluble CD14 fragment.
Claims18
A soluble CD14 antigen which has the following characteristic features 1) to 3):
- 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions;
- 2) an amino acid sequence in which the amino acid sequence of SEQ ID NO:1 is present on its N terminal;
- and
- 3) ability to specifically bind to an antibody prepared by using the peptide comprising 16 amino acid residues described in SEQ ID NO:2 for the antigen.
A recombinant soluble CD14 fragment produced by the following steps i) to iii):
- i) the step of producing a fragment having a partial sequence of the amino acid sequence described in SEQ ID NO:
- 3 in which a sequence of a cleavage site for a predetermined protease has been substituted or inserted, or a fragment having such partial sequence in which 1 to 10 amino acids have been deleted, added, or substituted in the region other than positions 53 to 68 of SEQ ID NO:
- 3;
- ii) the step of cleaving the recombinant soluble CD14 fragment produced in i) with the predetermined protease;
- and
- iii) the step of recovering the fragment of the N terminal side cleaved in ii);
- and
- having the following characteristic features 1) to 3):
- 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions;
- 2) no ability to specifically bind to 3C10 or MEM-18;
- and
- 3) ability to specifically bind to an antibody prepared by using the peptide comprising 16 amino acid residues described in SEQ ID NO:2 for the antigen.
A recombinant soluble CD14 fragment which has the following characteristic features 1) to 3):
- 1) a molecular weight of 13±2 kDa when measured by SDS-PAGE under non-reducing conditions;
- 2) no ability to specifically bind to 3C10 and MEM-18;
- and
- 3) ability to specifically bind to an antibody prepared by using the peptide comprising 16 amino acid residues described in SEQ ID NO:2 for the antigen.
Dependencies are inferred from the claim text.