Micro RT-PCR apparatus and method using the same
OwnerGwo-Bin Lee, Chia-Sheng Liaoinventors
Life of this patent
Estimating the term…
Abstract
A micro RT-PCR apparatus includes a chip module and control module. The chip module includes a reaction chamber unit, and a heating unit for heating the reaction chamber unit. The control module controls the heating unit to perform a first heating operation and a second heating operation. The first heating operation provides the reaction chamber unit with a temperature required to carry out a reverse transcription reaction. The second heating operation provides the reaction chamber unit with a temperature required to perform a polymerase chain reaction. An RT-PCR method using the micro RT-PCR apparatus is also disclosed.
Claims17
A micro reverse transcription-polymerase chain reaction (RT-PCR) apparatus, comprising:
- a chip module including a reaction chamber unit, and a heating unit for heating said reaction chamber unit,
- a control module including a temperature control unit for controlling said heating unit to perform a first heating operation and a second heating operation, said first heating operation providing said reaction chamber unit with a temperature required to carry out a reverse transcription (RT) reaction, said second heating operation providing said reaction chamber unit with a temperature required to perform a polymerase chain reaction (PCR).
An RT-PCR method comprising:
- using a chip module which has a reaction chamber unit and a heating unit for heating said reaction chamber unit;
- carrying out an RT reaction and a PCR reaction in said reaction chamber unit;
- and
- controlling said heating unit to perform a first heating operation and a second heating operation, said first heating operation providing said reaction chamber unit with a temperature required to carry out the RT reaction, said second heating operation providing said reaction chamber unit with a temperature required to perform the PCR reaction.
Dependencies are inferred from the claim text.